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Literature summary for 3.1.3.24 extracted from

  • Whitaker, D.P.
    Purification and properties of sucrose-6-phosphatase from Pisum sativum shoots (1984), Phytochemistry, 23, 2429-2430.
No PubMed abstract available
Search for more literature for 3.1.3.24

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Pisum sativum
maltose 100 mM, 27% inhibition Pisum sativum
sucrose 100 mM, 9% inhibition Pisum sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
 sucrose 6-phosphate
-
 Pisum sativum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Km: 0.175 mM Pisum sativum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 2 * 55000, SDS-PAGE Pisum sativum
115000
-
 gel filtration Pisum sativum

Organism

Organism UniProt Comment Textmining
Pisum sativum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pisum sativum

Source Tissue

Source Tissue Comment Organism Textmining
shoot
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pisum sativum

Storage Stability

Storage Stability Organism
-20°C, 10 mM HEPES buffer, pH 7, 50% glycerol, 1 mM dithiothreitol, stable Pisum sativum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl phosphate + H2O 2.5% of the activity with sucrose 6-phosphate Pisum sativum 4-nitrophenol + phosphate
-
 ?
sucrose 6-phosphate + H2O
-
 Pisum sativum sucrose + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 55000, SDS-PAGE Pisum sativum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
 Pisum sativum